Production of Monoclonal Antibody against Prokaryotically Expressed G1 Protein of Bovine Ephemeral Fever Virus

Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)

Bovine Viral Diarrhea virus (BVDV) is an important viral pathogen of cattle causing several clinical syndromes. There are usually no pathognomonic clinical signs of BVDV infection. Diagnostic investigations therefore rely on serological detection and virus isolation. Nonstructural protein 3 (NS3) as immunogenic protein of BVDV is genetically and antigenically conserved among different isolates....

Isolation of Bovine Ephemeral Fever Virus in Iran

Expression of G1- epitope of bovine ephemeral fever virus in E. coli : A novel candidate to develop ELISA kit

Bovine ephemeral fever is an acute and arthropod-borne viral disease of cattle and water buffalo which occurs seasonally in most of the world tropical and subtropical regions. The epizootic feature of the disease has been reported in Iran with serious economic consequences. The surface glycoprotein G of bovine ephemeral fever virus (BEFV) is composed of 4 antigenic sites (G1-G4) and plays the m...

Biopanning of polypeptides binding to bovine ephemeral fever virus G1 protein from phage display peptide library

BACKGROUND The bovine ephemeral fever virus (BEFV) glycoprotein neutralization site 1 (also referred as G1 protein), is a critical protein responsible for virus infectivity and eliciting immune-protection, however, binding peptides of BEFV G1 protein are still unclear. Thus, the aim of the present study was to screen specific polypeptides, which bind BEFV G1 protein with high-affinity and inhib...

Production of monoclonal antibody against recombinant NS3 protein of bovine viral diarrhea virus (NADL strain)

This study was conducted to investigate the prevalence of subclinical mastitis caused by Staphylococcus spp. in ewes in West-Azerbaijan province of Iran. Molecular characterization of isolated Staphylococcus spp. from diseased ewes were performed using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) and DNA sequencing of glyceraldehyde-3-phosphate deh...

Fluorescent antibody studies on ephemeral fever virus.

THEODORIDIS, A. Fluorescent antibody studies on ephemeral fever virus. Onderstepoort J. vet. Res., 36 (2) , 187-190, 1969. The preparation and use of a highly wecific fluorescein-conjugated antiserum against bovine ~phemeral fever virus are described. The demonstration of fluorescent cytoplasmic inclusions IS a dependable diagnostic test. The test also revealed cross reactions between the vir u...